Extractive Spectrophotometric Determination of Miconazole Nitrate in Pure and Pharmaceutical Preparation

	Extractive Spectrophotometric Determination of Miconazole Nitrate in Pure and Pharmaceutical Preparation
Article 3, Volume 1, Issue 4, Summer 2013, Page 246-252 PDF (506 K)
Document Type: Original Article
Abstract
A simple extractive spectrophotometric method has been developed for the estimation of miconazole nitrate in both pure and pharmaceutical preparation. The method is based on the formation of ion-pair complex of the drug with acidic dye bromothymol blue (BTB) in acidic condition, followed by its extraction in organic solvent (chloroform). The absorbance was measured against the corresponding blank. The maximum absorbance was found at 434 nm. Linear calibration graph was obtained in the concentration range 1-35 μg ml-1. The method was validated statistically. Recovery studies gave satisfactory results indicating that none of common additives and excipients interfere the assay method. The proposed methods were found to be simple, accurate nd reproducible and their performance in analysis of cream formulation was successfully examined.
Keywords
Miconazole nitrate; Extractive spectrophotometry; Bromothymol blue; Ion-pair complex


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